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Fig. 3. Size selection increases viral DNA read counts for cassava samples. (A) The average percent reads mapping to viral <t>DNA</t> <t>(ACMV</t> and <t>EACMCV)</t> and host DNA, and unmapped reads for total DNA (grey) and size-selected DNA (blue) samples. (B) The average reads per million (RPM) corresponding to ACMV DNA-A (AA), ACMV DNA-B (AB), EACMCV DNA-A (EA), and EACMCV DNA-B (EB) for total (grey) and size-selected (blue) DNA samples. (C) The average RPM corresponding to ACMV DNA-A (AA), ACMV DNA-B (AB) for virion DNA (yellow). Data in A, B, and C are from analyses of reads from li braries prepared from virus-infected plant DNA subjected to optimized RCA. The bars in A, B and C correspond to 2 standard errors from two biological replicates and two technical replicates each. The asterisks indicate a significant difference (P-value < 0.05) between the viral read counts in size-selected and total DNA in Student’s t-tests.
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Fig. 3. Size selection increases viral DNA read counts for cassava samples. (A) The average percent reads mapping to viral DNA (ACMV and EACMCV) and host DNA, and unmapped reads for total DNA (grey) and size-selected DNA (blue) samples. (B) The average reads per million (RPM) corresponding to ACMV DNA-A (AA), ACMV DNA-B (AB), EACMCV DNA-A (EA), and EACMCV DNA-B (EB) for total (grey) and size-selected (blue) DNA samples. (C) The average RPM corresponding to ACMV DNA-A (AA), ACMV DNA-B (AB) for virion DNA (yellow). Data in A, B, and C are from analyses of reads from li braries prepared from virus-infected plant DNA subjected to optimized RCA. The bars in A, B and C correspond to 2 standard errors from two biological replicates and two technical replicates each. The asterisks indicate a significant difference (P-value < 0.05) between the viral read counts in size-selected and total DNA in Student’s t-tests.

Journal: Journal of virological methods

Article Title: An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing.

doi: 10.1016/j.jviromet.2021.114405

Figure Lengend Snippet: Fig. 3. Size selection increases viral DNA read counts for cassava samples. (A) The average percent reads mapping to viral DNA (ACMV and EACMCV) and host DNA, and unmapped reads for total DNA (grey) and size-selected DNA (blue) samples. (B) The average reads per million (RPM) corresponding to ACMV DNA-A (AA), ACMV DNA-B (AB), EACMCV DNA-A (EA), and EACMCV DNA-B (EB) for total (grey) and size-selected (blue) DNA samples. (C) The average RPM corresponding to ACMV DNA-A (AA), ACMV DNA-B (AB) for virion DNA (yellow). Data in A, B, and C are from analyses of reads from li braries prepared from virus-infected plant DNA subjected to optimized RCA. The bars in A, B and C correspond to 2 standard errors from two biological replicates and two technical replicates each. The asterisks indicate a significant difference (P-value < 0.05) between the viral read counts in size-selected and total DNA in Student’s t-tests.

Article Snippet: Infectious clones of ACMV and EACMCV (Addgene plasmids 159134 to 159137) were a gift from Vincent Fondong (Delaware State University).

Techniques: Size Selection, Virus, Infection